Primer Dilution

How To Dilute Your Primers

If you have a primer in solution that is 100 micromolar, that is the same as 100 nmol/ml or 100 pmol/µl. We only need 12 picomoles in one sequencing reaction so dilute in the following method:

  1. Do a 1:10 dilution, take 10 µl of 100 µM solution add 90 µl of water. This will give you a solution of 10 pmol/µl.
  2. Add 1.2 µl of primer to the sequencing mix you supply to Core Facilities to provide us with 12 picomoles.